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In Order To Obtain The Optimal ELISA Results

1．Please carefully reconstitute Standards or working Detection Reagent A&B according to the instruction and avoid foaming and mix gently until the crystals have completely dissolved;

2．Proper adhesion of plate sealers during incubation steps is necessary;

3．Substrate, Detection Reagent A&B should be incubate for 30 min at 37℃ before use;

4．After the every step of aspiration / wash, please do not delay entering into the next step in order not to let the assay plate "too dry".

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