1.Please carefully reconstitute Standards or working Detection Reagent A&B according to the instruction and avoid foaming and mix gently until the crystals have completely dissolved;
2.Proper adhesion of plate sealers during incubation steps is necessary;
3.Substrate, Detection Reagent A&B should be incubate for 30 min at 37℃ before use;
4.After the every step of aspiration / wash, please do not delay entering into the next step in order not to let the assay plate "too dry".